Statistics for mtSNPs:
Validation for V-MitoSNP designed ready-for-use primers (natural and mismatch) for mtSNPs:
Supplementary Fig. 1.The mtSNP primer validation for V-MitoSNP. To validate the designed primers in V-MitoSNP, the corresponding natural and/or mismatched primers for mtSNPs at position 8993, 5973, 7080, 12372, 15508, and 8829 of rCRS are tested by PCR. Lane 1, 4, 7, 10, 13, and 16 is 100bp DNA marker. The brightest band is 500bp and the band in bottom is 100bp. Primer sets for 8993, 5973 and 7080 are natural primers which are shown in duplicate at lane 2/3, lane 5/6, and lane 8/9, while for 12372, 15508, and 8829 are mismatched primers which are shown in duplicate at lane 11/12, lane 14/15 and lane 17/18, respectively.Detail PCR information is provided on line by range input.PCR was performed with the following protocol: 94°C (1 min); 4 cycles of 94°C (15s), 64°C (15 s), 70°C (15 s); 4 cycles of 94°C (15 s), 61°C (15 s), 70°C (15 s); 4 cycles of 94°C (15 s), 58°C (15 s), 70°C (15 s); 60 cycles of 94°C for (15 s), 55°C (15 s), 70°C (15 s); 94°C (1 min) and 60°C (5 min). The PCR results are confirmed by 1.5% agarose electrophoresis.
Please use the range input for each position to get the primers and enzyme information on-line if needed.