Non-RFLP primer design

Input mode:

Please input reference cluster ID (rs#) to obtain SNP flanking sequence for PCR-CTPP primer design

SNP flanking sequence (sense trand by default)

Organism: Human Mouse Rat

Database:

Sequence length: Select sequence length: Input sequence length:

Reference cluster ID(rs#):
example: rs12947788 or 12947788(Human), rs13465319 or 13465319 (Mouse), rs8148912 or 8148912 (Rat)

Please input reference cluster ID (rs#) or SNP sequence for PCR-CTPP primer design

The results report

1. Online 2. By email 3.Both online and by email

*Email address to receive the results:

Advanced options

Primer design constraints
Primer length: bps ~ bps	default (18 bps~ 26 bps)
Primer length difference: under bps	default (5 bps)
Tm (melting temperature): °C ~ °C	default (45°C ~ 62 °C )
Tm (melting temperature) difference: under °C	default (1.0 °C)
Na+ (molar sodium concentration): M	default (0.5 M)
GC proportion: % ~ %	default (20 % ~ 80 %)

Annealing check (Blast selection only is highly suggested to get informative primer design.)
GC clamp	Dimer check
Hairpin check	Blast for specificity