Mutagenic primer design

Input mode:

Please input reference cluster ID (rs#) to obtain SNP flanking sequence for mutagenic RFLP primer design

SNP flanking sequence (sense trand by default)

Organism: Human Mouse Rat

Database:

Sequence length: Select sequence length: Input sequence length:

Reference cluster ID(rs#):
example: rs11540652 or 11540652 (Human), rs13465322 or 13465322 (Mouse), rs8152226 or 8152226 (Rat)

Please input SNP sequence or get SNP sequence from above for mutagenic RFLP primer design

The results report

1. Online 2. By email 3.Both online and by email

*Email address to receive the results:

Advanced options

Primer design constraints
Primer length: bps ~ bps	default (18 bps~ 26 bps)
Primer length difference: under bps	default (5 bps)
Tm (melting temperature): °C ~ °C	default (50.0 °C ~ 62.0 °C)
Tm (melting temperature) difference: under °C	default (5.0 °C)
Na+ (molar sodium concentration): M	default (0.5 M)
GC proportion: % ~ %	default (40 % ~ 60 %)
PCR length: bps ± bps	default (200 bps ± 20 bps)
The range for mutagenic base from SNP: ± bps	default (5 bps)