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General PD
Natural PD
Mutagenic PD
CTPP PD
User Manual
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Input mode:
General primer design from sequence file
Sequence file:
The results report
1. Online
2. By email
3.Both online and email
*
Email address to receive the results:
Advanced options
Primer design constraints
Primer length:
bps ~
bps
default (18 bps~ 26 bps)
Primer length difference: under
bps
default (5 bps)
Tm (melting temperature):
°C ~
°C
default (50.0 °C ~ 62.0 °C)
Tm (melting temperature) difference: under
°C
default (5.0 °C)
Na+ (molar sodium concentration):
M
default (0.5 M)
GC proportion:
% ~
%
default (40 % ~ 60 %)
PCR length:
bps ±
bps
default (200 bps ± 20 bps)
Annealing check
GC clamp
Dimer check
Hairpin check
Blast for specificity
kuas&kmu©2006 design by yuhuei
