Introduction
General PD
Natural PD
Mutagenic PD
CTPP PD
User Manual
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Input mode:
Please input the sequence for general primer design
GTTCTCAATA CCGGGAGAGG CACAGAGCTA TTTCAGCCAC ATGAAAAGCA TCGGAATTGA GATCGCAGCT CAGAGGACAC CGGGCGCCCC TTCCACCTTC CAAGGAGCTT TGTATTCTTG CATCTGGCTG CCTGGGACTT CCCTTAGGCA GTAAACAAAT ACATAAAGCA GGGATAAGAC TGCATGAATA TGTCGAAACA GCCAGTTTCC AATGTTAGAG CCATCCAGGC AAATATCAAT ATTCCAATGG GAGCCTTTCG GCCAGGAGCA GGTCAACCCC CCAGAAGAAA AGAATGTACT CCTGAAGTGG AGGAGGGTGT TCCTCCCACC TCGGATGAGG AGAAGAAGCC AATTCCAGGA GCGAAGAAAC TTCCAGGACC TGCAGTCAAT CTATCGGAAA TCCAGAATAT TAAAAGTGAA CTAAAATATG TCCCCAAAGC TGAACAGTAG TAGGAAGAAA AAAGGATTGA TGTGAAGAAA TAAAGAGGCA GAAGATGGAT TCAATAGCTC ACTAAAATTT TATATATTTG TATGATGATT GTGAACCTCC TGAATGCCTG AGACTCTAGC AGAAATGGCC TGTTTGTACA TTTATATCTC TTCCTTCTAG TTGGCTGTAT TTCTTACTTT ATCTTCATTT TTGGCACCTC ACAGAACAAA TTAGCCCATA AATTCAACAC CTGGAGGGTG TGGTTTTGAG GAGGGATATG ATTTTATGGA GAATGATATG GCAATGTGCC TAACGATTTT GATGAAAAGT TTCCCAAGCT ACTTCCTACA GTATTTTGGT CAATATTTGG AATGCGTTTT AGTTCTTCAC CTTTTAAATT ATGTCACTAA
The results report
1. Online
2. By email
3.Both online and by email
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Email address to receive the results:
Advanced options
Primer design constraints
Primer length:
bps ~
bps
default (18 bps~ 26 bps)
Primer length difference: under
bps
default (5 bps)
Tm (melting temperature):
°C ~
°C
default (50.0 °C ~ 62.0 °C)
Tm (melting temperature) difference: under
°C
default (5.0 °C)
Na+ (molar sodium concentration):
M
default (0.5 M)
GC proportion:
% ~
%
default (40 % ~ 60 %)
PCR length:
bps ±
bps
default (200 bps ± 20 bps)
Annealing check
GC clamp
Dimer check
Hairpin check
Blast for specificity
kuas&kmu©2006 design by yuhuei
